Future University of Egypt

Assistant Lecturer at the Future University of Egypt

(FUE) and NODCAR {National Organization for Drug

Control and Research}. Reviewer at Bioscience reports

journal, Biochemical Society (neuroscience). Eight years’

experience as QA&QC. Highly trained and qualified to

deal/work on: Mesenchymal stem cells homing and

developing techniques, PCR, western blotting technique,

electrophoresis, Elisa, spectrophotometer, HPLC, dissolution

and disintegration. Successfully trained & passed the QMS

ISO 9001:2008 documentation, implantation training &

internal audit.

Neurocognitive disorders have been characterized by being a devastating

long-term neurological disease with a huge social and economic impact.

Alzheimer's disease (AD) is the most common type of dementia accompanied

with decline in cognitive functions. Objectives: The main aim of this study is to

suggest therapeutic protocol having the potentials for restoring and modifying

neuropathological deposited hallmarks including both positive and negative

lesions such as amyloid plaques, tau protein and synaptic loss. Materials and

Methods :Rats were divided into nine groups:(G1) control;(G2) rats received

lipopolysaccharide (LPS) as a method of inducing nongenetically manipulated

neuroinflammatory AD type ;(G3) AD rats received NaHS; (G4) AD rats received

mesenchymal stem cells (MSCs) intracerebrally; (G5)AD rats received

MSCs+NaHS ; (G6) AD rats received kefir+Ginko Biloba (GB); (G7) AD rats received

MSCs+ kefir+GB;(G8)AD rats received NaHS+kefir+GB; (G9)AD rats received

MSCs+NaHS+kefir+GB. Results: The induction of AD resulted in downregulation of

cystathionine β-synthase enzyme (CBS) relative gene expression and Glutathione

(GSH) brain tissue level accompanied with overexpression in amyloid-β

protein, mitogen-activated protein kinases (MAPK), tau protein, ACAT(Acyl-

CoA:cholesterol Acyl transferase) relative gene expression and Malondialdehyde

(MDA) brain tissue level in addition to elevated caspase-3 serum activity level.

Conclusion: The present study clearly proved the beneficial role of NaHS as

exogenous H2S donor in attenuating AD drawbacks mainly through CBS/H2S

overexpression enhancing the degradation of Aβ deposited plaques in addition to

the ability of MSCs administrated locally to develop into different types of neural

cells to compensate damaged ones. Regarding the role of administrating Kefir

and GB in maintaining normal brain functions suggested to be as a result of their

synergistic antioxidant and anti-apoptotic actions

Neurological Disorders and Stroke